Review Expression profiling in cancer using cDNA microarrays
نویسندگان
چکیده
Currently there are over 1 000 000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50±90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique.
منابع مشابه
The impact of expression profiling on prognostic and predictive testing in breast cancer.
Expression profiling has been extensively applied to the study of breast cancer and undoubtedly is changing the way breast cancer is perceived. Over the past few years, several groups have described prognostic "signatures" (gene lists) that are purported to be more accurate prognostic factors than well established clinical and pathological features. In addition, cDNA and oligonucleotide microar...
متن کاملGene expression profiling of alveolar rhabdomyosarcoma with cDNA microarrays.
Several forms of human sarcoma, lymphoma, and leukemia are characterized by somatically acquired chromosome translocations that result in fusion genes that encode chimeric transcription factors with oncogenic properties. We have used cDNA microarrays containing 1238 cDNAs to investigate the gene expression profile of a group of seven alveolar rhabdomyosarcoma (ARMS) cell lines characterized by ...
متن کاملCombination of PCR subtraction and cDNA microarray for differential gene expression profiling.
PCR subtraction hybridization has been used effectively to enrich and single out differentially expressed genes. However identification of these genes by means of cloning and sequencing individual cDNAs is a tedious and lengthy process. In this report, an attempt has been made to combine the use of PCR select cDNA subtraction hybridization and cDNA microarrays to identify differentially express...
متن کاملDiscrimination of Drug Sensitivity of Cancer Using cDNA Microarray and Multivariate Statistical Analysis
Practical diagnosis of complex diseases, e.g. cancer, requires high throughput profiling of gene expression in tissues. Currently, the most promising technology for it is microarrays [1]. In our method, each cDNA from a cDNA library is spotted (target) at one segment on glass-plates (4108*double/plate), with which mixture of cDNAs from normal cell mRNAs and cancer cell mRNAs (each is labeled wi...
متن کاملGene expression profiling reveals overexpression of TSPAN13 in prostate cancer.
Prostate cancer is one of the most frequent malignancies in the Western world. The identification of additional molecular markers is needed to refine the diagnosis of prostate cancer and to develop more effective therapies. In order to identify molecular abnormalities involved in prostate cancer progression, we performed gene expression analysis of prostate cancer samples compared to matched no...
متن کامل